Technology - Genetic Engineering
Reporter Gene Technique For Imaging Cell Kinetics
Cells desired to be monitored are genetically engineered in culture by transfection, transduction or electroporation/nucleofection to constitutively express the reporter gene and then administered into the living subject. At any desired time-point thereafter a specific imaging probe can be injected to image reporter gene expression to determine cell kinetics. This technique can be used to image all aspects of cell kinetics.